DNA-Protein Interactions > Cell Biology

The interaction of proteins with DNA is of critical importance in modern biology, with major roles, for example, in defining and regulating chromosome structure, DNA replication, recombination and repair, gene transcription, and viral infection.
This book is a unique compendium of the many methods now available for studying the specific interactions of proteins with DNA. Its contents reflect the fact that this is a rapidly advancing research area in which a multidisciplinary approach is essential for solving problems and obtaining a detailed understanding of the molecular regulatory networks in which many of these interactions are involved. The book describes many of the major techniques now available, with particular emphasis on biophysical approaches to the analysis of transcription and its relationship to chromatin structure. Each approach is described in full detail by authors whose practical experience has guided the crucial steps to success. The result is a book that is essential reading for all researchers currently engaged in studying protein: DNA interactions, or who plan to do so.

Expression Systems
Gel electrophoresis and bending in cisplatin-modified linear DNA
Use of DNA microcircles in protein-DNA binding studies
Use of topology to measure protein-induced DNA bend and unwinding angles
Bandshift, gel retardation, or electrophoretic mobility shift assays
Practical aspects of Fluorescence Resonance Energy Transfer (FRET) and its applications in nucleic acid biochemistry
Determination of DNA-ligand interactions by fluorescence correlation spectroscopy
DNA wrapping in E. Coli RNA polymerase open promotor complexes revealed by scanning force microscopy
Microcalorimetry of protein-DNA interactions
DNA-protein cross-linking by formaldehyde: a probe for RNAP-open promoter complex structure
Solid-phase DNAse I footprinting
Hydroxyl radical footprinting
Radiolytic cleavage of DNA: Mapping of the Protein Interaction Sites
UV - Laser photoreactivity of nucleoprotein complexes in vitro
In vivo UV-laser footprinting
Digitization and quantitative analysis of footprinting gels
Mapping histone positions in chromatin by protein-directed DNA cross-linking and cleavage
Kinetics of DNA-protein interactions
Kinetics of DNA interactions studied by surface plasmon resonance spectroscopy
Quantitative DNase I Kinetics Footprinting
Real time fluorescence anisotropy for studying protein-DNA interactions
Analysis of protein-DNA interactions in complex nucleoprotein assemblies
Site-specific protein-DNA photocrosslinking
Visualization of protein-DNA complexes by cryo em and on grids
Characterization of T7 RNA polymerase protein-DNA interactions during the initiation and elongation phases

등록된 상품문의

상품문의 쓰기 새 창 더보기

상품문의가 없습니다.